6EK7

YaxA from Yersinia enterocolitica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	203.760, 24.050, 109.420
Unit cell angles	90.00, 113.92, 90.00

Refinement procedure

Resolution	14.950 - 1.800
R-factor	0.1912
Rwork	0.190
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet derivative of YaxA
RMSD bond length	0.009
RMSD bond angle	0.941
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmeas	0.055	0.550
Number of reflections	45510
<I/σ(I)>	11.5	2
Completeness [%]	97.8	97.6
Redundancy	3.7
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M LiCl; 40% MPD