6EK1
Crystal structure of Type IIP restriction endonuclease PfoI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 48.067, 48.067, 266.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.219 - 2.601 |
| R-factor | 0.2233 |
| Rwork | 0.218 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.574 |
| Data reduction software | MOSFLM (6.2.6) |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | MOLREP (9.2.10) |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.220 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.112 | 0.440 |
| Rpim | 0.074 | |
| Number of reflections | 17795 | 1470 |
| <I/σ(I)> | 5.7 | 3.4 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 5.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 6.8 mg/ml of PfoI in complex with DNA oligoduplex was mixed with reservoir solution 1:1, microseeding was used. NaHepes 0.1M, magnesium formate 0.2M, 18-20% PEG3350 |
