6EJT
Nuclease NucB from Bacillus licheniformis in P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98148 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 23.228, 47.821, 100.234 |
Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
Resolution | 47.820 - 1.700 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ejs |
RMSD bond length | 0.016 |
RMSD bond angle | 1.759 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.820 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.031 | 0.090 |
Rmeas | 0.037 | 0.114 |
Rpim | 0.026 | 0.078 |
Number of reflections | 22140 | 963 |
<I/σ(I)> | 21.8 | 8.3 |
Completeness [%] | 91.1 | 75.2 |
Redundancy | 3.1 | 2.7 |
CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 1.8 M ammonium sulphate, 0.1 M sodium acetate pH 4.6, soaked in 0.02 M 3'-AMP |