6EJS
Nuclease NucB from Bacillus licheniformis in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.9,0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.534, 47.414, 56.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.280 - 1.600 |
R-factor | 0.1921 |
Rwork | 0.188 |
R-free | 0.24000 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.778 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.410 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.037 | 0.116 |
Rmeas | 0.043 | 0.142 |
Rpim | 0.022 | 0.079 |
Number of reflections | 10804 | 448 |
<I/σ(I)> | 29.8 | |
Completeness [%] | 90.1 | 77.7 |
Redundancy | 6.7 | 5.9 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 2.2 M ammonium sulphate, 0.1 M Tris pH 8.5 |