6EJQ
Crystal structure of a C-terminally truncated small terminase protein from the thermophilic bacteriophage G20c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2015-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 167.275, 167.275, 139.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.470 - 2.300 |
| R-factor | 0.2094 |
| Rwork | 0.208 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xvn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.115 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.470 | 49.470 | 2.340 |
| High resolution limit [Å] | 2.300 | 12.390 | 2.300 |
| Rmerge | 0.126 | 0.029 | 1.811 |
| Rmeas | 0.131 | 0.031 | 1.883 |
| Rpim | 0.035 | 0.010 | 0.511 |
| Total number of observations | 1187820 | 6769 | 59925 |
| Number of reflections | 88169 | 670 | 4451 |
| <I/σ(I)> | 16.1 | 65.4 | 1.6 |
| Completeness [%] | 100.0 | 98.4 | 100 |
| Redundancy | 13.5 | 10.1 | 13.5 |
| CC(1/2) | 0.999 | 0.999 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 35% v/v Tacsimate pH 7.0 |
