6EJN

The KLC2 TPR domain bound to the JIP3 leucine zipper domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 65
Unit cell lengths	163.301, 163.301, 77.123
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.710 - 3.200
R-factor	0.192
Rwork	0.191
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ceq
RMSD bond length	0.009
RMSD bond angle	1.120
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.5.7)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.123	77.123	3.370
High resolution limit [Å]	3.200	10.120	3.200
Rmerge		0.024	1.591
Rmeas	0.113	0.026	1.698
Rpim	0.039	0.009	0.579
Total number of observations	152770	4650	22427
Number of reflections	19393	631	2821
<I/σ(I)>	13.8	59.3	1.6
Completeness [%]	99.5	97.4	99.8
Redundancy	7.9	7.4	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	20% PEG 3350 0.2 M NaCSN