6EJN
The KLC2 TPR domain bound to the JIP3 leucine zipper domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 65 |
| Unit cell lengths | 163.301, 163.301, 77.123 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.710 - 3.200 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ceq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.123 | 77.123 | 3.370 |
| High resolution limit [Å] | 3.200 | 10.120 | 3.200 |
| Rmerge | 0.024 | 1.591 | |
| Rmeas | 0.113 | 0.026 | 1.698 |
| Rpim | 0.039 | 0.009 | 0.579 |
| Total number of observations | 152770 | 4650 | 22427 |
| Number of reflections | 19393 | 631 | 2821 |
| <I/σ(I)> | 13.8 | 59.3 | 1.6 |
| Completeness [%] | 99.5 | 97.4 | 99.8 |
| Redundancy | 7.9 | 7.4 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 20% PEG 3350 0.2 M NaCSN |
