6EJL
Structure of 14-3-3 zeta in complex with ASK1 14-3-3 binding motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-10 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.3418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.980, 93.427, 68.491 |
Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
Resolution | 30.322 - 2.382 |
R-factor | 0.1966 |
Rwork | 0.196 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qjb |
RMSD bond length | 0.008 |
RMSD bond angle | 0.894 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.010 | 2.530 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.077 | 0.277 |
Number of reflections | 18601 | 2937 |
<I/σ(I)> | 20.57 | 7.15 |
Completeness [%] | 99.4 | 97.8 |
Redundancy | 6.96 | 6.89 |
CC(1/2) | 0.999 | 0.976 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Morpheus HT-96, condition H12 |