6EJI

Structure of a glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	123
Detector technology	PIXEL
Collection date	2012-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	61.620, 127.030, 71.580
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	29.942 - 2.300
R-factor	0.197
Rwork	0.196
R-free	0.22340
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.950	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.130	1.234
Number of reflections	179312
<I/σ(I)>	4.39	2.59
Completeness [%]	89.1	98
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, potassium iodide, mops