6EJH
Crystal structure of the G343C mutant of Candida albicans Mep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.700, 104.700, 160.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.400 - 1.710 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5af1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.113 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rpim | 0.041 | 0.340 |
| Number of reflections | 70763 | 5289 |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 4.7 |
| CC(1/2) | 0.993 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Tris pH 8 28% PEG 400 |
