6EJH
Crystal structure of the G343C mutant of Candida albicans Mep2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 |
Unit cell lengths | 104.700, 104.700, 160.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.400 - 1.710 |
R-factor | 0.1725 |
Rwork | 0.171 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5af1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.113 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.400 | 1.750 |
High resolution limit [Å] | 1.710 | 1.710 |
Rpim | 0.041 | 0.340 |
Number of reflections | 70763 | 5289 |
<I/σ(I)> | 11.9 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 4.7 |
CC(1/2) | 0.993 | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Tris pH 8 28% PEG 400 |