6EJ6
Crystal structure of the N240A mutant of Candida albicans Mep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.623, 104.623, 160.974 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.800 - 1.650 |
| R-factor | 0.1787 |
| Rwork | 0.178 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5af1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.800 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.039 | 0.483 |
| Number of reflections | 77396 | 5527 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 84.4 | 80.1 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Tris pH 8 30% PEG400 |
