6EI6
CC2D1B coordinates ESRCT-III activity during the mitotic reformation of the nuclear envelope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.410, 54.050, 97.900 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 59.594 - 2.461 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.24750 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.216 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.594 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.072 | 0.360 |
| Number of reflections | 33151 | |
| <I/σ(I)> | 6 | 3.6 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.995 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1 M Bis-Tris pH 6.5, 15-25% PEG 3500 Mme, 200 mM ammonium sulfate |
