6EI3
Crystal structure of auto inhibited POT family peptide transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2013-09-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96859 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.060, 65.230, 70.340 |
| Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
| Resolution | 24.650 - 2.100 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aps |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.650 | 24.650 | 2.150 |
| High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
| Rmerge | 0.097 | 0.032 | 0.686 |
| Rmeas | 0.103 | 0.034 | 0.774 |
| Rpim | 0.035 | 0.012 | 0.354 |
| Total number of observations | 3551 | 12301 | |
| Number of reflections | 36377 | 416 | 2655 |
| <I/σ(I)> | 15.2 | 51.9 | 2.5 |
| Completeness [%] | 99.9 | 94.6 | 99.9 |
| Redundancy | 8.2 | 8.5 | 4.6 |
| CC(1/2) | 0.999 | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.5 | 292 | 100 mM TrisHCl, 120 mM ammonium tartrate, 20 %(v/v) PEG 400, pH 8.5 |
