6EHY
scFv AbVance: increasing our knowledge of antibody structural space to enable faster and better decision making in drug discovery
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-02 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.061, 71.040, 121.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.250 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.28500 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.060 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 20323 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 99.5 |
| Redundancy | 5.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 4.5M NaCl 100mM HEPES pH 7.5 |
