6EHY

scFv AbVance: increasing our knowledge of antibody structural space to enable faster and better decision making in drug discovery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-02
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	48.061, 71.040, 121.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.680 - 2.250
R-factor	0.231
Rwork	0.228
R-free	0.28500
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.060
High resolution limit [Å]	2.250
Number of reflections	20323
<I/σ(I)>	6.2
Completeness [%]	99.5
Redundancy	5.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	4.5M NaCl 100mM HEPES pH 7.5