6EHV

scFv AbVance: increasing our knowledge of antibody structural space to enable faster and better decision making in drug discovery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92
Spacegroup name	I 41 2 2
Unit cell lengths	132.899, 132.899, 80.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.840 - 2.540
R-factor	0.276
Rwork	0.275
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.370
High resolution limit [Å]	2.540
Number of reflections	12186
<I/σ(I)>	10.1
Completeness [%]	99.6
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2M ammonium sulphate