6EHR
The crystal structure of the human LAMTOR-RagA CTD-RagC CTD complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0723 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.090, 92.448, 127.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.224 - 2.898 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.26260 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.419 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.224 | 3.070 |
High resolution limit [Å] | 2.898 | 2.900 |
Rmerge | 0.156 | 0.933 |
Rpim | 0.078 | 0.478 |
Number of reflections | 21957 | |
<I/σ(I)> | 8.2 | 1.4 |
Completeness [%] | 93.8 | 93.4 |
Redundancy | 4.4 | |
CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% [w/v] PEG 4000, 0.1M tri-sodium-citrate pH5.5, 5% [v/v] isopropanol |