6EHF
OmpT (in-vitro folded), an outer membrane protein Vibrio cholerae (trimer form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-05-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 200.910, 200.910, 200.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.030 - 2.720 |
| R-factor | 0.24015 |
| Rwork | 0.238 |
| R-free | 0.28059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ehd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.910 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.030 | |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rpim | 0.025 | 0.679 |
| Number of reflections | 18714 | |
| <I/σ(I)> | 22.1 | 1.2 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 42.2 | 43.7 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 0.05 M calcium chloride dihydrate, 0.05 M barium chloride, 0.1 M tris, 32% PEG 400 |
