6EHB
OmpU, an outer membrane protein, of Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.723, 153.840, 66.170 |
| Unit cell angles | 90.00, 102.32, 90.00 |
Refinement procedure
| Resolution | 49.490 - 1.550 |
| R-factor | 0.17172 |
| Rwork | 0.170 |
| R-free | 0.19929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3upg |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rpim | 0.037 | 0.555 |
| Number of reflections | 171689 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.900 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M sodium acetate, 0.1 M MES, 28% w/v 400 PEG |
