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6EH8

HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04-1
Synchrotron siteDiamond
BeamlineI04-1
Temperature [K]100
Detector technologyPIXEL
Collection date2012-02-09
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.917
Spacegroup nameP 1 21 1
Unit cell lengths69.210, 49.500, 72.590
Unit cell angles90.00, 94.66, 90.00
Refinement procedure
Resolution52.080 - 2.510
R-factor0.2266
Rwork0.223
R-free0.29590
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4gkz
RMSD bond length0.011
RMSD bond angle1.552
Data reduction softwarexia2
Data scaling softwareSCALA (3.3.20)
Phasing softwarePHASER
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]52.08452.0842.580
High resolution limit [Å]2.51011.2302.510
Rmerge0.0301.388
Rmeas0.1470.0401.706
Rpim0.0730.0210.834
Number of reflections16674
<I/σ(I)>12.420.70.4
Completeness [%]98.297.797.9
Redundancy3.83.63.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP29115% PEG 4K, 15% Glycerol, 100mM MES pH 7.0

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