6EH8
HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.917 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.210, 49.500, 72.590 |
Unit cell angles | 90.00, 94.66, 90.00 |
Refinement procedure
Resolution | 52.080 - 2.510 |
R-factor | 0.2266 |
Rwork | 0.223 |
R-free | 0.29590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gkz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.552 |
Data reduction software | xia2 |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.084 | 52.084 | 2.580 |
High resolution limit [Å] | 2.510 | 11.230 | 2.510 |
Rmerge | 0.030 | 1.388 | |
Rmeas | 0.147 | 0.040 | 1.706 |
Rpim | 0.073 | 0.021 | 0.834 |
Number of reflections | 16674 | ||
<I/σ(I)> | 12.4 | 20.7 | 0.4 |
Completeness [%] | 98.2 | 97.7 | 97.9 |
Redundancy | 3.8 | 3.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% PEG 4K, 15% Glycerol, 100mM MES pH 7.0 |