6EH4

003 Human T-Cell Receptor specific for HIV GAG epitope SLYNTVATL carried by Human Leukocyte Antigen HLA-A*0201

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	43.230, 81.220, 65.070
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	43.230 - 1.260
R-factor	0.16008
Rwork	0.159
R-free	0.19068
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gkz
RMSD bond length	0.023
RMSD bond angle	2.219
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.230
High resolution limit [Å]	1.260
Number of reflections	106284
<I/σ(I)>	15.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	25% PEG 4K, 15% Glycerol, 100mM TRIS pH 8.0