6EGR
Crystal structure of Citrobacter freundii methionine gamma-lyase with V358Y replacement
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-22 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.667, 122.872, 127.515 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.240 - 1.450 |
R-factor | 0.1401 |
Rwork | 0.138 |
R-free | 0.17380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.844 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 | 1.280 |
High resolution limit [Å] | 1.200 | 1.450 | 1.200 |
Rmerge | 0.434 | ||
Rmeas | 0.614 | 7.124 | |
Rpim | 0.434 | ||
Number of reflections | 249456 | 45054 | |
<I/σ(I)> | 7.18 | 2 | |
Completeness [%] | 93.3 | 99.7 | 76.9 |
Redundancy | 2.1 | ||
CC(1/2) | 0.837 | 0.117 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 35% MMEPEG 2000, 50 MM TRIS-HCL, 0.2 MM PLP, PROTEIN 10 MG/ML, 0.25% DTT, PH 8.5 |