6EGK
T181N Cucumene Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.330, 91.810, 124.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.480 - 1.960 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.769 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.480 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.133 | 1.020 |
| Rpim | 0.058 | 0.468 |
| Number of reflections | 47590 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 97.5 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.996 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Bis-Tris, 12% PEG monomethyl ether 5000 |
