6EG5
The structure of SB-1-202-tubulin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97937 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.541, 158.033, 181.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.450 |
R-factor | 0.19761 |
Rwork | 0.196 |
R-free | 0.23628 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ezy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.352 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.148 | 1.200 |
Number of reflections | 111555 | |
<I/σ(I)> | 28.5 | 2 |
Completeness [%] | 100.0 | |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6% POLYETHYLENE GLYCOL 4000, 8% GLYCEROL, 0.1 M MES, 30 MM CACL2, 30 MM MGCL2, PH 6.7 |