6EFZ
Crystal Structure of DIP-Theta Ig1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | .979 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 79.103, 146.476, 189.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.925 - 3.499 |
| R-factor | 0.244 |
| Rwork | 0.243 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eg1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.000 | 3.830 |
| High resolution limit [Å] | 3.499 | 3.499 |
| Rmerge | 0.060 | 0.556 |
| Number of reflections | 14202 | 3287 |
| <I/σ(I)> | 0.06 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 12% PEG4000, 0.3 M americium sulfate, 20% glycerol, 0.08 M sodium acetate, pH 4.6, cryoprotectant: 30% glycerol |
