6EFY
Crystal Structure of DIP-Alpha Ig1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .979 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 104.578, 104.578, 102.195 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.938 - 2.900 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eo9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.693 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.780 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.784 |
| Number of reflections | 14611 | 2349 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 2% PEG3350, 17% Tacsimate, pH 7.0, 0.1 M HEPES, pH 7.0, cryoprotectant: 30% glycerol |
