6EFN
Structure of a RiPP maturase, SkfB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.462, 83.110, 61.299 |
Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
Resolution | 42.574 - 1.291 |
R-factor | 0.1445 |
Rwork | 0.142 |
R-free | 0.16850 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.927 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.574 | 1.340 |
High resolution limit [Å] | 1.291 | 1.291 |
Number of reflections | 92230 | |
<I/σ(I)> | 43.6 | 1.8 |
Completeness [%] | 96.7 | 76.2 |
Redundancy | 5.6 | 2.6 |
CC(1/2) | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 294.15 | 0.25 M magnesium formate, 15% PEG3350 |