6EFI
SK678 binding region (Siglec + Unique)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.624, 59.579, 61.832 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 38.718 - 1.715 |
| R-factor | 0.1822 |
| Rwork | 0.181 |
| R-free | 0.21170 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 45715 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 97.7 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M bicine pH 7.6, 25% PEG 6000, 0.005 M hexamine Cobalt chloride |
