6EFC

Hsa Siglec + Unique domains (unliganded)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	46.635, 58.173, 75.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.452 - 1.400
R-factor	0.1472
Rwork	0.145
R-free	0.17900
RMSD bond length	0.009
RMSD bond angle	1.174
Refinement software	PHENIX ((1.14_3211))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.400	1.400
Number of reflections	39091
<I/σ(I)>	49.7
Completeness [%]	93.3
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M SPG pH 10.0, 25% PEG3350