6EFA

GspB Siglec + Unique domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	32.980, 47.630, 136.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.034 - 1.600
R-factor	0.1673
Rwork	0.163
R-free	0.21160
RMSD bond length	0.017
RMSD bond angle	1.604
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Number of reflections	29333
<I/σ(I)>	43.8
Completeness [%]	88.9
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium sulfate, 25% PEG 3350