6EET
Crystal structure of mouse Protocadherin-15 EC9-MAD12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 129.507, 170.059, 91.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.810 - 3.230 |
| R-factor | 0.17854 |
| Rwork | 0.176 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bxz 4xhz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 (v717) |
| Data scaling software | HKL-2000 (v717) |
| Phasing software | PHASER (v2.7.0) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.166 | 0.808 |
| Number of reflections | 16630 | 785 |
| <I/σ(I)> | 14.529 | 2.222 |
| Completeness [%] | 99.5 | 94.8 |
| Redundancy | 9.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES sodium salt, 30% (w/v) MPD, 5% (w/v) PEG4000 |
