6EER
Structure of glycine-bound GoxA from Pseudoalteromonas luteoviolacea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.242, 93.464, 187.903 |
Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
Resolution | 48.295 - 1.820 |
R-factor | 0.1774 |
Rwork | 0.175 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6byw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 1.850 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.068 | 0.405 |
Rpim | 0.062 | 0.365 |
Number of reflections | 338105 | 16759 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 292 | 22% w/v PEG 3350, 0.1 M ammonium sulfate, 0.1 M HEPES pH 7.5 mixed 1:1 with protein at 10 mg/mL |