6EEP

Crystal Structure of Ribose-5-phosphate Isomerase from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	104.630, 67.930, 74.000
Unit cell angles	90.00, 119.54, 90.00

Refinement procedure

Resolution	32.190 - 1.850
R-factor	0.1613
Rwork	0.158
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x84
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX ((1.14_3219))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.190	32.190	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.058	0.032	0.548
Rmeas	0.067	0.037	0.626
Total number of observations	155837
Number of reflections	37853	442	2820
<I/σ(I)>	13.97	31.97	2.54
Completeness [%]	97.7	95.5	97.3
Redundancy	4.117	3.647	4.242
CC(1/2)	0.998	0.997	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	LepnA.00944.a.B1.PS38423 at 22.85 mg/ml was mixed 1:1 with MCSG1(B8): 25.5% (w/v) PEG-4,000, 15% (v/v) Glycerol, 0.085 M Tris Base / Hydrochloric, 0.17 M Sodium Acetate, pH = 8.5. Tray: 300294b8. Puck: jve-8