6EEG
Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-08-12 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.037, 60.508, 38.184 |
| Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.950 |
| R-factor | 0.1746 |
| Rwork | 0.172 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.959 |
| Data reduction software | APEX 2 |
| Data scaling software | SHELXPREP |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.120 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.026 | 0.124 |
| Number of reflections | 10364 | 456 |
| <I/σ(I)> | 27.46 | 7.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.79 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
