6EDX
Crystal Structure of SGK3 PX domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | I 4 |
Unit cell lengths | 74.339, 74.339, 51.963 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.590 - 2.009 |
R-factor | 0.1907 |
Rwork | 0.187 |
R-free | 0.21280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xte |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.590 | 2.060 |
High resolution limit [Å] | 2.009 | 2.009 |
Rmerge | 0.122 | 0.181 |
Rpim | 0.035 | 0.051 |
Number of reflections | 9533 | 682 |
<I/σ(I)> | 13.9 | 9.1 |
Completeness [%] | 99.9 | |
Redundancy | 13.3 | |
CC(1/2) | 0.997 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M Sodium Citrate, 2M Sodium Malonate (pH 5.0) |