6EDX

Crystal Structure of SGK3 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	I 4
Unit cell lengths	74.339, 74.339, 51.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.590 - 2.009
R-factor	0.1907
Rwork	0.187
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xte
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.590	2.060
High resolution limit [Å]	2.009	2.009
Rmerge	0.122	0.181
Rpim	0.035	0.051
Number of reflections	9533	682
<I/σ(I)>	13.9	9.1
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	0.997	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Sodium Citrate, 2M Sodium Malonate (pH 5.0)