6EDQ

Crystal Structure of the Light-Gated Anion Channelrhodopsin GtACR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	77.790, 149.550, 62.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.910 - 2.900
R-factor	0.2317
Rwork	0.230
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eid
RMSD bond length	0.005
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.910	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	16711	7958
<I/σ(I)>	4.54
Completeness [%]	99.8
Redundancy	20
CC(1/2)	0.994	0.317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	295	15% MPD, 100 mM ADA, pH 6.0, and 100 mM NaCl