6EDM
Structure of apo-CDD-1 beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 123.201, 123.201, 123.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 1.400 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.18120 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmeas | 0.071 | 0.949 |
| Rpim | 0.020 | 0.265 |
| Number of reflections | 63142 | |
| <I/σ(I)> | 19.3 | 2.7 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 12.8 | 12.4 |
| CC(1/2) | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES, pH 7.0, 3.0 M ammonium sulfate |
