6EDK

Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis with N10-formyltetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	97.200, 77.300, 40.460
Unit cell angles	90.00, 103.90, 90.00

Refinement procedure

Resolution	47.180 - 1.800
R-factor	0.1706
Rwork	0.168
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ci2
RMSD bond length	0.017
RMSD bond angle	1.353
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.180	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.063	0.763
Rpim	0.027
Number of reflections	26389	1627
<I/σ(I)>	12.2
Completeness [%]	97.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M ammonium sulfate, 0.1M MES pH 6.5, 26% PEG5000 MME