6EDE

tRNA 2'-phosphotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 61 2 2
Unit cell lengths	53.284, 53.284, 308.393
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.145 - 1.553
R-factor	0.1735
Rwork	0.173
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e3a
RMSD bond length	0.005
RMSD bond angle	0.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.550	1.550
Rpim	0.014	0.208
Number of reflections	39099
<I/σ(I)>	56.6	2.8
Completeness [%]	99.3	85.8
Redundancy	17.7
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M MES pH 6.0, 10 %MPD