6ED2

Faecalibacterium prausnitzii beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03
Spacegroup name	P 64 2 2
Unit cell lengths	145.232, 145.232, 233.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.850 - 2.300
R-factor	0.1726
Rwork	0.172
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.850	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	67984
<I/σ(I)>	19.48
Completeness [%]	99.6
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Bis-Tris Propane: HCl, pH 7 0.4 M Magnesium Formate