6ED1
Bacteroides dorei Beta-glucuronidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 242.663, 101.355, 168.600 |
Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
Resolution | 29.536 - 2.900 |
R-factor | 0.1804 |
Rwork | 0.178 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uj6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.059 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.118 | 0.417 |
Number of reflections | 90006 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.3 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.35 M sodium citrate, 16% PEG3350 |