6ECW

StiD O-MT residues 956-1266

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 41
Unit cell lengths	71.086, 71.086, 123.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.532 - 1.700
R-factor	0.1698
Rwork	0.169
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.532	46.532	1.800
High resolution limit [Å]	1.700	5.060	1.700
Rmerge	0.084	0.031	2.098
Rmeas	0.087	0.032	2.179
Total number of observations	932033
Number of reflections	67485	2605	10835
<I/σ(I)>	19.77	74.59	1.22
Completeness [%]	99.9	99.7	99.7
Redundancy	13.811	13.443	13.666
CC(1/2)	1.000	1.000	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 M ammonium citrate tribasic pH 7.2