6ECV

StiD O-MT residues 976-1266

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1
Unit cell lengths	40.182, 56.175, 72.251
Unit cell angles	86.31, 84.89, 75.81

Refinement procedure

Resolution	42.494 - 1.798
R-factor	0.1684
Rwork	0.167
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.494	42.494	1.910
High resolution limit [Å]	1.798	5.360	1.800
Rmerge	0.073	0.050	0.667
Rmeas	0.087	0.058	0.792
Number of reflections	54842	2102	8699
<I/σ(I)>	9.92	24.56	1.82
Completeness [%]	96.6	97.3	94.5
Redundancy	3.531	3.423	3.467
CC(1/2)	0.996	0.996	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2 M NaF