6ECN

HIV-1 CA 1/2-hexamer-EE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	65.600, 84.100, 248.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.400
R-factor	0.22789
Rwork	0.225
R-free	0.27533
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.012
RMSD bond angle	1.599
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (7.0.0)
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.100	3.490
High resolution limit [Å]	3.400	3.400
Rmerge	0.130	1.100
Number of reflections	36208
<I/σ(I)>	5.7
Completeness [%]	99.0
Redundancy	3.1
CC(1/2)	0.997	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.1M Sodium Citrate pH 5, 8% PEG 8000