6ECM

Crystal Structure of SNX15 PX domain in domain swapped conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	54.498, 54.498, 83.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.353
R-factor	0.2499
Rwork	0.244
R-free	0.29890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ikd
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.076	1.074
Rpim	0.015	0.216
Number of reflections	5645	505
<I/σ(I)>	20.9	3.1
Completeness [%]	99.4	94.3
Redundancy	26.2	24.6
CC(1/2)	1.000	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris (pH 8.5) 200 mM LiSO4, 24% PEG4000, 10% glycerol