6ECI
Structure of the FAD binding protein MSMEG_5243 from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Xenocs GeniX 3D Cu HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 80.268, 186.039, 195.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.599 - 2.690 |
| R-factor | 0.2117 |
| Rwork | 0.209 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fhk |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.740 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.215 | 1.444 |
| Rmeas | 0.231 | 1.552 |
| Rpim | 0.084 | 0.565 |
| Number of reflections | 82043 | 4447 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.3 |
| CC(1/2) | 0.993 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291.15 | 20% polyethylene glycol 1500, 4% 2-methyl-2,4-pentanediol and 0.1 M citric acid pH 3.5 |
