6EBT

Crystal structure of recombinant mutant N107T of human fumarase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 65 2 2
Unit cell lengths	188.816, 188.816, 114.145
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	93.597 - 2.300
R-factor	0.152
Rwork	0.150
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5upp
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1-2575-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.100	2.370
High resolution limit [Å]	2.300	2.300
Number of reflections	53547
<I/σ(I)>	12.4
Completeness [%]	100.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	0.2 M HEPES 16% (m/v) PEG 10000