6EBA

Crystal Structure of A Bacterial Homolog to Human Lysosomal Transporter, Spinster, in Inward-facing And Unoccupied Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	87.628, 98.487, 125.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.244 - 3.812
R-factor	0.3155
Rwork	0.315
R-free	0.32570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e8j
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.244	3.907
High resolution limit [Å]	3.812	3.812
Number of reflections	11062
<I/σ(I)>	11.98
Completeness [%]	99.0
Redundancy	6.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	32.5%(v/v) PEG 400, 100 mM Tris-HCl