6EB9
Crystal Structure of the Nipah Virus Phosphoprotein Multimerization Domain Delta 542-544
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 45.502, 45.502, 102.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.260 - 1.900 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.982 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.260 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.240 |
| Rpim | 0.030 | 0.260 |
| Number of reflections | 17906 | 1529 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.2 | 94.6 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | Protein at 12 mg/ml in 300mM NaCl and 10 mM Tris pH 8 mixed 1:1 with 10% (v/v) 2-propanol, 0.1 M sodium citrate tribasic dihydrate pH 5.0, and 26% (v/v) polyethylene glycol 400 |
