6EB6

Crystal structure of BAX W139A monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 31 2 1
Unit cell lengths	63.159, 63.159, 82.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.870 - 2.023
R-factor	0.1729
Rwork	0.171
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w61
RMSD bond length	0.007
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.550	2.095
High resolution limit [Å]	2.023	2.023
Rmerge	0.063	0.526
Number of reflections	12611	1200
<I/σ(I)>	21.15
Completeness [%]	98.4
Redundancy	10.1
CC(1/2)	0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium formate, TCEP