6EAZ

Apo structure of the mitochondrial calcium uniporter protein MICU2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	291
Detector technology	PIXEL
Collection date	2015-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	112.562, 125.524, 72.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.803 - 2.504
R-factor	0.201
Rwork	0.198
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.810	2.512
High resolution limit [Å]	2.500	2.504
Number of reflections	35943
<I/σ(I)>	14.8
Completeness [%]	98.2
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	4% PEG3350, 150 mM tri-lithium citrate