6EAE

CRYSTAL STRUCTURE OF HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION GLYCOPROTEIN INHIBITOR ESCAPE VARIANT L141W STABILIZED IN THE PREFUSION STATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2016-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 41 3 2
Unit cell lengths	167.190, 167.190, 167.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.370 - 2.900
R-factor	0.2002
Rwork	0.199
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c69
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.410	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.160	1.743
Rmeas	0.165	1.792
Rpim	0.039	0.412
Total number of observations	323166
Number of reflections	18328	2903
<I/σ(I)>	14
Completeness [%]	100.0	100
Redundancy	17.6	18.7
CC(1/2)	0.999	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.44M K/Na tartrate, 0.2M LiSO4, 0.1M CHES pH 9.5